CBMR Postdoc Position – Proteomics, Bioinformatics, Biotechnology, Biology, Diabetes Metabolism is available at Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
Postdoc in Proteomics and Bioinformatics
Deadline to Apply
Apr 19, 2021 (GMT+1)
No. of Position(s)
According to Standard Norms
Novo Nordisk Foundation Center for Basic Metabolic Research
Faculty of Health and Medical Sciences
Starting date is October 1, 2021.
- PhD in Biology or related sciences
- Excellent scientific track record in relation to career stage
- Knowledge concerning sample preparation for proteomics and phosphoproteomics analysis
- Knowledge concerning proteomics data analysis using Python, R or other programming languages
- Ability to analyse raw MS files using MaxQuant, Spectronaut, Skyline or similar platforms
- Willingness to implement new relevant methods for proteomics analysis
- Excellent English communication skills, both written and oral
- Hands-on experience using liquid chromatography and tandem mass spectrometry on Thermo Scientific instruments with standard software instrument control
- Experience with targeted proteomics (SRM, MRM)
- Experience with isobaric labelling (preferentially TMT)
- Certified with FELASA laboratory animal science
- Candidate with additional background in metabolism, diabetes and the measurement of proteins and posttranslational modifications within skeletal muscle would be highly regarded
The CBMR International Postdoctoral Program is open for applicants that hold a PhD degree awarded from a university outside of Denmark. The PhD degree has to be obtained before October 1, 2021. The program is also open for applicants with a Danish PhD degree who can document at least 12 months full-time research experience from outside Denmark. You are not eligible to apply for the program if you have been employed in a postdoctoral position for more than one year at the University of Copenhagen prior to the commencement of the fellowship.
- The overarching aim of this research project is to identify and understand subcellular organelle level protein dynamics of insulin stimulated skeletal muscle.
- We hypothesize that insulin regulates skeletal muscle metabolism through compartmentalization of proteins and phosphoproteins.
- Your task will be to apply mass spectrometry-based proteomics to map insulin-induced changes in organellar proteome and phosphoproteome in skeletal muscle from control and diet-induced insulin-resistant mice.
- The results from the proteomics analysis will be validated by high-resolution microscopy in collaboration with the microscopy facility at the University of Copenhagen.
- You are expected to
1) perform organelle fractionation on skeletal muscle tissue
2) prepare samples for proteomics and phosphoproteomics analysis
3) perform advanced bioinformatics analysis to study organelle proteomics and phosphoproteomics
4) perform high-resolution microscopy in collaboration with the core facility.
How to Apply?
To apply, please click APPLY ONLINE button mentioned above
- Cover letter expressing the motivation and previous research experience of the applicant (max 1 page)
- Curriculum vitae
- Copy of the PhD degree certificate and the Master degree certificate. In case the PhD has not yet been completed, a written statement from the supervisor confirming it will be obtained before October 1, 2021 is acceptable.
- List of publications
- References (name and contact details of at least two references)
About the Project
The current position is in the Program for Integrative Physiology and Environmental Influences. The candidate will be part of the Functional Proteomics in Metabolism Group headed by Associate Professor Atul Deshmukh and work in close collaboration with the Novo Nordisk Foundation Center for Protein Research at the University of Copenhagen. The successful candidate will work to integrate proteomics and functional physiology, to answer basic and applied research questions related to physical exercise, diet and metabolism. He or she will be embedded within the Center and work in a scientific environment that bridges several disciplines of proteomics and metabolic diseases. Our facilities include state-of-the-art laboratories and core facilities and the position provides unique opportunities to advance expertise in state-of-the-art quantitative mass spectrometry and data analysis of large datasets. Striving for academic excellence, collegial respect and freedom tempered by responsibility, we offer creative, friendly and stimulating working conditions in a dynamic and strong scientific environment with the opportunity to plan work independently to further professional and personal development.
Skeletal muscle is the largest tissue in the human body and plays an important role in locomotion and whole body metabolism. It accounts for ~80% of insulin stimulated glucose disposal. Skeletal muscle insulin resistance, a primary feature of Type 2 diabetes, is a consequence of defects in intracellular signalling leading to failure in insulin-mediated effects on glucose and lipid metabolism. Insulin signalling diverges into multiple branches affecting every organelle of the cell, and for a subset of proteins, this involves translocation from one compartment to another (e.g. FOXO family proteins). This illustrates that insulin signalling is rather complex and dynamic. Identifying these dynamics in skeletal muscle will reveal novel insight into insulin signalling and how muscle organelles respond in health and disease.
- For further information about the position, please contact Associate Professor Atul Deshmukh at email@example.com. Questions regarding the CBMR International Postdoctoral Program must be directed to firstname.lastname@example.org.
- For the relevant literature and technical details, please refer to these articles PMIDs: 16615899, 30352176, and 27278775.
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Post expires at 8:59am on Tuesday April 20th, 2021 (GMT+9)