Postdoctoral Position in Mass Spectrometry liquid chromatography, Proteomics

    Postdoctoral Position in Mass Spectrometry liquid chromatography, Proteomics

    Mass Spectrometry liquid chromatography, Proteomics – Postdoctoral Position in the area of fundamental mass spectrometry/proteomics, in the Department of Biochemistry and Molecular Biology at the University of Southern Denmark


    Deadline to Apply

    May 17, 2021 (23: 59 GMT +1)


    Overview

    PositionPostdoctoral Position
    No. of Position(s)1
    Research Area– Mass Spectrometry
    – Proteomics 
    SalaryAccording to standard norms
    WorkplaceDepartment of Biochemistry and Molecular Biology
    University of Southern Denmark
    Denmark
    Contract Period2 Years
    Starting dateMay 17, 2021

    Qualifications

    • Apply if you have substantial experience with mass spectrometry-based proteomics/metabolomics and chromatographic separation.
    • This project implies close collaboration and knowledge sharing between group members and collaborators. The successful candidate should also be able to work independently and have comprehensive knowledge of solution-phase and gas-phase chemistry of biomolecules.
    • This means that you have at least two scientific publications supporting mass spectrometry skills of biomolecules. The documented experience and mentioning of the scientific publications should be stated in the cover letter.

    Preference

    • Advanced knowledge of data-processing and analysis (R scripting) is an advantage.
    • In addition, a strong background in one or more of the following fields: analytical chemistry, protein chemistry, cell biology, biochemistry, or a related field as well as excellent exams are a prerequisite for application submission
    • Applicants are expected to be highly motivated, reliable and interested in working in an interdisciplinary and international environment. Finally, a good command of English is a requirement. 

    Responsibilities/ Job Description

    The successful applicant will be responsible for investigating new settings of liquid chromatography and mass spectrometry ideal for detection of negatively charged peptides. This includes systematic testing of mobile phases, column materials, proteolytic enzymes, and MS parameters. You will be using this setup to characterize and validate a number of peptide-specific features, e.g. peptide retention times, and develop in collaboration, machine-learning and statistical tools that ensures reliable protein identification and quantification. The developed methodology will be tested in combination with traditional positive ion-mode proteomics on a number of biological relevant samples.

    Furthermore, it is expected that the successful candidate will participate in the supervision and teaching of undergraduate students.

    How to Apply?

    • Applications must be submitted electronically using the link “Apply online”. Attached files must be in Adobe PDF or Word format.
    • Each box can only contain a single file of max. 10 Mb.
    • We strongly recommend that you read How to apply before you apply.

    Documents Required

    • A curriculum vitae including information on previous employment.
    • A full list of publications stating the scientific publications on which the applicant wishes to rely.
    • At least one recommendation letter. Should your referees wish to send their letters directly to us, please have them use e-mail: anc@sdu.dk mentioning your name and the title of the position in the subject line. And please note that these also need to reach us before deadline.

    About Research project

    Current proteomics approaches lack complete protein coverage largely due to the almost exclusive use of positive ion mode as the ionization method, which results in inefficient ionization of many acidic peptides. With the existence of equal amount of acidic and alkaline proteins (the same distribution for their derived peptides) in the human proteome this inefficient ionization poses a significant challenge, yet at the same time presents substantial potential. This fact underlines the timeliness to advance the area of proteomics by making use of the largely unexplored negative ion mode.

    Scientific environment

    The Kjeldsen research group is part of the Protein Research Group (PRG) at the University of Southern Denmark (SDU) in Odense, Denmark. Odense is located about one hour from the main capital of Denmark, Copenhagen. PRG is internationally recognized for its contributions to biological mass spectrometry, proteomics, biomedical research and clinical proteomics. Research is spanning from fundamental gas-phase chemistry to biomedical studies, including protein structural analysis, cancer and neuroscience.

    Today the PRG consists of eight individual research groups encompassing more than 50 scientists, postdocs and students who share facilities and research environment. The PRG has a fantastic student environment with many multicultural inputs. The PRG has the newest mass spectrometers for biological mass spectrometry applications including Orbitrap Eclipse, Orbitrap Exploris, and TIMS-TOF-PRO. In addition, the PRG has a fully equipped protein chemistry/proteomics laboratory, cell laboratory facilities and bioinformatics that will be available for the project.

    Note

    Further information for international applicants about entering and working in Denmark.

    Inquiries

    Frank Kjeldsen
    Email: frankk@bmb.sdu.dk .

    Official advertisement

    Postdoctoral Position in Mass Spectrometry-based Proteomics (Method Development)

    Post expires at 8:59am on Tuesday May 18th, 2021 (GMT+9)